First of all, the eggs of the expectant mother are collected and kept in the laboratory for a while, then the eggs are peeled and the mature cells are separated. The aim here is to determine the mature eggs to be used for microinjection. Afterwards, the sperms in the semen sample taken from the male are prepared by washing method and injected into each mature egg with special devices called micromanipulators. Then, it is monitored at 24-hour intervals in special cabinets called incubators where internal environment is adjusted and constantly observed. Meanwhile, the egg injected with sperm is stored in special liquids called medium. The success rate of this procedure depends on the preparation of the couple, the conditions of the embryology laboratory and, of course, a teamwork of the experienced embryologist and nurse crew.